A 23-year-old woman was raped in the toilets of Orchard Reloaded nightclub in Hazlemere in the early hours of Friday (14/11) morning.
The woman was raped in the male toilets of the club, in Inkerman Hill, at around 3.15am.
Detective sergeant Robin Hughes, investigating officer, said: "I urge anyone who witnessed the incident or has any information that may help us with our investigation, to contact me at High Wycombe police station on 0845 8 505 505.
“I am particularly keen to hear from anyone who was in the male toilets of Orchard Reloaded at around 3.10am.”
Police are also appealing for witnesses to a related incident of criminal damage outside the night club, involving a blue Volkswagen Golf and a dark blue Subaru Impreza, at approximately 3.30am.
If you don’t want to talk to police and don’t want to leave your name call the Crimestoppers charity on 0800 555 111.